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| Lopez Flores, L; Suarez Santiago, V; Longet, D; Saulnier, Denis; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and... |
| Tipo: Text |
Palavras-chave: Molecular phylogeny; Actin gene; Ostrea edulis parasite; Bonamia ostreae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3562.pdf |
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| Certad, Gabriela; Zahedi, Alireza; Gantois, Nausicaa; Sawant, Manasi; Creusy, Colette; Duval, Erika; Benamrouz-vanneste, Sadia; Ryan, Una; Viscogliosi, Eric. |
| Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be... |
| Tipo: Text |
Palavras-chave: Piscine Cryptosporidium; Edible marine fish; Genetic characterization; 18S rDNA gene; Actin gene; Molecular phylogeny. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77741/79864.pdf |
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| Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar. |
| An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010 |
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